The volume of spheroids that stepwise cell number seeding, is quantified by using Cell3iMager Estier.
MCF7 cells (RIKEN BRC)
DMEM (Nacalai tesque)
96-well plate U bottom (Sumitomo Bakelite)
75 - 300 cells/well
3 days
Low magnification lens
Each scan 3 um pitch
Median 3D filter (X, Y, Z radius; 2.0, 2.0, 2.0)
3D Object Counter v2.0(Threshold; 8000)