Materials and Methods
- Cell Line: HepG2 cells (RIKEN BRC)
- Medium: DMEM (Nacalai tesque)
- Plate for 2D culture: 96-well plate flat bottom (Corning)
- Plate for 3D culture: 96-well plate flat bottom ULA (Corning)
- Seeding cell density: 0 - 2000 cells/well
- Culture days: 4 days
- Reagent: Hypoxia probe LOX-1(Organogenix)
- Imaging methods: Fluorescence, Low magnification lens
Cell3iMager duos is very useful for cell counting.
It is possible to analysis for various fluorescent regents and other cells.