2D cell culture | Confluency | Label free assay

Confluency

Objective

The cell density that stepwise cell number seeding, is quantified by using Cell3iMager duos.

Materials and Methods

  • Cell Line: HepG2 cells (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • Plate: 96-well plate flat bottom (Sumitomo Bakelite)
  • Seeding cell density: 0 - 10000 cells / well
  • Culture days: 4 days
  • Imaging methods: Bright field, Low magnification lens

Results and Conclusions

  • Cell3iMager duos is possible to clearly measure the cells around a edge of well on label-free assay.
  • The data indicated that the density is depended manner of the seeding cell number.

 

 

2D cell culture | Confluency | Label free assay                2D cell culture | Confluency | Label free assay

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