Colony formation | Colony formation units assay | Crystal violet
Summary
Cell3iMager neo is useful for 'colony formation assay' stained by crystal violet.
Materials and Methods
Cell Line: HCT116 cells (RIKEN BRC)
Medium: DMEM (Nacalai tesque)
Reagent: 1% Crystal violet (Wako)
Plate: 6-well plate f lat bottom (IWAKI)
Seeding cell density: 50 cells / well
Culture days: 14 days
Imaging methods: 4800 dpi
Results and Conclusions
It is possible to the quantification of colony formation assay using 'the area SUM', 'the number of colonies' and 'average diameter of colony' is easy.
The numerical data can be displayed as a heat map. It can be visually confirmed the difference between samples.
