Cell3iMager Estier  is a versatile stage-top optical coherence tomography (OCT) instrument that allows non-invasive analysis of the 3D structures including spheroids, organoids, tubular structures and tissues etc.

3D Live -Cell Imaging using Infra red laser Tomography

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3D ex vivo imaging  -  Tumor Invasion - Cell3iMager Estier (cc-9000) Preclinical ex vivo imaging platform

Estier is a stage-top optical coherence tomography (OCT) instrument that allows non-invasive analysis of the 3D structures including microvessels/tubular structures, tissues, spheroids and organoids. As OCT provides an advantageous platform to visualize the in vitro and ex vivo dynamics of the 3D structures conveniently, it can be used as an alternative visualization/monitoring method to obtain quantitative information about the morphological changes occurring in 3D cell culture samples.

  • Estier OCT technology enables lable-free and non-invasive tomographic imaging and monitoring of microvessels, neovasculature formation to quantify and evaluate morphological changes in a time lapse manner. Ideal for tumor invasion assays and evaluation of  efficacy of molecules, such as, Avastin which is a well known anti-angiogeneic drug.
  • The ability to measure true volume (X,Y and Z directions) of 3D structures (Spheroids/Organoids) enables a true comparison of drug efficacy  in preclinical models, e.g., in vitro/ex vivo platforms using Patient derived cells versus xenografts. 
  • Detects necrotic regions and quantifies volume, internal cavities, tubular structures etc., with impressive focus
  • A cost-effective supplementary system to an existing imaging platform
  • 3D ex vivo imaging - Tumor Invasion - Estier is a stage-top optical coherence tomography (OCT) instrument that allows non-invasive analysis of the 3D structures including microvessels/tubular structures, tissues, spheroids and organoids. As OCT provides an advantageous platform to visualize the in vitro and ex vivo dynamics of the 3D structures conveniently, it can be used as an alternative visualization/monitoring method to obtain quantitative information about the morphological changes occurring in 3D cell culture samples.
  • 3D ex vivo imaging  - Tumor Invasion -  Estier OCT technology enables lable-free and non-invasive tomographic imaging and monitoring of microvessels, neovasculature formation to quantify and evaluate morphological changes in a time lapse manner. Ideal for evaluating for efficacy of anti-angiogeneic molecules, such as Avastin.
  • 3D ex vivo imaging - The ability to measure true volume (X,Y and Z directions) of 3D structures (Spheroids/Organoids) enables a true comparison of drug efficacy  in preclinical models, e.g., in vitro/ex vivo platforms using Patient derived cells versus xenografts.
    Skin test
  • 3D ex vivo imaging - Allows differentiation of sample’s by detecting the image contrast originating from variances in the sample’s physical density (refractive index (RI))
    Skin test
3D ex vivo imaging
Non-invasive deep tissue imaging
  • Enable non-invasive detection of internal cavities and gapsin tissues (up to 1 mm thick).
  • A 300 μm2 3D image can be acquired in 1 minute.
  • High-resolution (3 μm) and low resolution (10 μm) imaging options (range of focus 1μm-10 μm) with accurate focus options
in vitro Drug-screening
Easy operation
  • User friendly work-flow.
  • No special trainings and technical expertise are required for operating the system and conducting image analysis.
  • Allows differentiation of sample’s by detecting the image contrast originating from variances in the sample’s physical density (refractive index (RI))
Bright-field imaging
User friendly analysis software
  • Dedicated software facilitates fast true 3D data acquisition and image reconstruction.
  • Simple and straightforward user interface that require no extensive operational training or expertise
System compatibility
  • Any standard cell cultureware such as micro well plates, petri dishes etc., can be used.
  • The system can be integrated into any existing work-flow.
  • No special labware or reagents are required.
3D ex vivo imaging - Estier is a stage-top optical coherence tomography (OCT) instrument that allows non-invasive analysis of the 3D structures including microvessels/tubular structures, tissues, spheroids and organoids. As OCT provides an advantageous platform to visualize the in vitro and ex vivo dynamics of the 3D structures conveniently, it can be used as an alternative visualization/monitoring method to obtain quantitative information about the morphological changes occurring in 3D cell culture samples.

1.  3D morphological analyses for cultures / internal structures with OCT

OCT  is a non-invasive technology  that can be used to acquire the tomogram of  live cell or organism  by using feeble near-infrared light.

  • OCT technology  enables a true 3D imaging and deep penetration into 3D structures including spheroids, tissues and microvessels. 
  • Enables researchers acquire 3D image in real time and simultenously ensuring that the structural intricacies of objects are preserved for longterm examination.
  • Ease of use and cost effective  technolgy for multiple therapeuic areas including Oncology, skin disorders and regenerative medicine.
  • 3D ex vivo imaging - Estier is a stage-top optical coherence tomography (OCT) instrument that allows non-invasive analysis of the 3D structures including microvessels/tubular structures, tissues, spheroids and organoids. As OCT provides an advantageous platform to visualize the in vitro and ex vivo dynamics of the 3D structures conveniently, it can be used as an alternative visualization/monitoring method to obtain quantitative information about the morphological changes occurring in 3D cell culture samples.
  • 3D ex vivo imaging - Estier is a stage-top optical coherence tomography (OCT) instrument that allows non-invasive analysis of the 3D structures including microvessels/tubular structures, tissues, spheroids and organoids. As OCT provides an advantageous platform to visualize the in vitro and ex vivo dynamics of the 3D structures conveniently, it can be used as an alternative visualization/monitoring method to obtain quantitative information about the morphological changes occurring in 3D cell culture samples.
    Ovary
     ​(Courtesy: Prof. Nobuo Nagai, Nagahama institute of Bio-Science and Technology)
  • 3D ex vivo imaging - Kidney  Tumor spheroids
    Kidney spheroid
     ​(Courtesy: Prof. Tetsuya Ohbayashi, Tottori University)
  • 3D ex vivo imaging - Neovasculature Tumor spheroids
    Neovasculature
     ​(Courtesy: Prof. Yukiko T. Matsunaga, University of Tokyo)
Collaborative research with Prof. Tamio Mizukami, Nagahama institute of Bio-Science and Technology
Collaborative research with Prof. Tamio Mizukami, Nagahama institute of Bio-Science and Technology

2. Time-lapse observation by non-invasive and label-free analysis

  • Real time observation from spheroid formation to collapse after addition of anti-cancer drug
  • Detailed observation for 3D structure of spheroids that conventional microscopes cannot recognize
  • 3D ex vivo imaging - Chetomin (10mM) addition
    Chetomin (10mM) addition
  • 3D ex vivo imaging
  • 3D ex vivo imaging
  • 3D ex vivo imaging
    Microscope

3. Compatibility for conventional labwares

  • Compatible with all standard micro well plates (SBS format) and culture dishes
  • Specific well plates optimized for 3D culture inclduding Corning® Elplasia® can be used for imaging 
  • SCREEN provides customized solutions for our researchers. These include implementing customized plate formats,  such as microfluidic chips, organ-on-chips. 
Features
Non-invasive : Observable in culture with general consumable.
Various samples : Available from “spheroid” to “biological tissue”.
Method
SD-OCT
Resolution
High resolution : 3um
Low resolution : 10um
Max. FOV
High resolution : 1×1mm
Low resolution : 10×10mm (Wide F.O.V.)
Obser­vation time (e.g.)
One cross section : 0.5s~
3D construction :
– High resolution 0.3×0.3×0.3mm/3um : 2min.
– Low resolution 5.0×5.0×1.0mm/10um : 9min.
Display Analysis
– Sectional image of any direction.
– 3D view image of any direction.
– Distance between arbitrary points.
– 2D sectional area / 3D volume.
Consumable
SBS-micro well plate, Culture dish, etc.
(Various consumables can be used with adapter.)
Components
– Main unit (W20×D20×H19 inch)
– Sub unit, spectrometer (W7×D18×H12 inch)
– PC(W7×D18×H17 inch) + mouse, key board, joy-stick
Cell3iMager Estier (cc-9000)
 3D Live -Cell Imaging using Infra red laser Tomography

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