2d cell culture - toxicology - Multi-fluorescence imaging 3D Cell Imaging: Cell3iMager Duos 2 Applications : ScreenLifeSience

3D Cell Culture by using FCeM®

Multi spheroids cultured in FCeM-medium were quantified by using Cell3iMager duos.

3D Cell Culture by using Matrigel®

Using Cell3iMager duos is effective for quantification of tumor spheroids volume cultured in Matrigel.

Evaluation of hypoxia level in 2D/3D-cultured cells

Hypoxia level of 2D/3D-cultured cells was evaluated by using Cell3iMager duos.

Analysis of microscope images

Microscopy images are possible to import into Cell3iMager duos. These images can be also analyze and measurement using Cell3iMager software.

Analysis of spheroids in microwells (EZSPHERE® plate)

Spheroids were cultured with EZSPHERE® PLATE and analyzed using Deep Learning plug-in.

Cell Proliferation

Proliferation ratio of the 2D/3D cultured cells is quantified by Cell3iMager duos.

Label free colony-forming units assay

We performed the labeled-free colony formation assay using Cell3iMager duos.

Confluency

The cell density that stepwise cell number seeding, is quantified by using Cell3iMager duos.

Counting fluorescence stained adherent cells

The cell density that stepwise cell number seeding, was quantyfied by using Cell3iMager duos.

Deep Learning for cell classification

Jarkat cells induced cell death by Anti-Fas antibody were classified by Cell3iMager duos and Deep Learning.

Cell Viability and Cytotoxicity Assay

Viability of anti-cancer drug treated cells is quantified by using Cell3iMager duos.

Tissue sections (H&E)

Using Cell3iMager duos, It is possible to image tissue sections on preparation by color. (Because Cell3iMager duos has the color image sensor.)

Single Cell Cloning Hybridoma

Cell 3imager DUOS imaging platform can be utilized for label free assessment of single cells, picking up the clone of interest and further assessment of the desired clone over a period of days. This allows monitoring the growth of single cell to a full fledged hybridoma colonies.