3D Cell Culture by Hanging Drop

3D Cell Culture by Hanging Drop

Spheroid | 3D culture | Hanging Drop | Label free assay

Summary

Spheroids in the droplets being aggregated by the method of hanging drop were quantified by Cell3iMager neo.

Materials and Methods

 
  • Cell Line
    • HepG2 cells (RIKEN BRC)
    • NIH3T3 cells (RIKEN BRC)
  • Medium: DMEM (Nacalai tesque)
  • Plate: GravityPLUS™ (InSphero)
  • Seeding cell density: 500, 1000cells/well
  • Culture days: 3 days after making drops
  • Imaging methods
    • Bright-field, 4800dpi
    • Bracket Focus (stacked)

Results and Conclusions

  • Cell3iMager neo could capture the spheroid formation process in hanging drops.
  • It was possible to quantify the spheroids to evaluate the time-lapse changing area.

                    

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